Geometric optimization methods for the analysis of gene expression data

Detection of gene expression and sequence analysis of chicken class II trans activator (CIITA)

BACKGROUND:Class II transactivator (CIITA) is a dominanttranscriptional element, controlling numerous genes in theimmune system. CIITA is expressed in a constitutive pattern inantigen presenting cells although its expression can occur inother cell types. Since the revelation of CIITA, there have beenconsiderable advances toward understanding its role as anactivator of MHC II genes in humans and...

P-77: Optimization of Ovine FSH Gene Expression in The Pichiapastoris System byRegulating The Culture Conditions

Background: Ovine follicle stimulation hormone (OFSH) is a pituitary glycoprotein and belongs to the family of glycoprotein hormones. This hormone plays a key role in the function of the reproductive system: it is essential for sertoli cell function and spermatogenesis in testis and it stimulates the growth of ovulatory follicles in females. Ovine FSH hormone is a heterodimeric hormone consisti...

short Communication: Optimization of semi-quantitative RT PCR analysis for CPT I gene expression in Rainbow trout (Oncorhynchus mykiss)

P-73: Effect of Donor Age on The Expression Stability of GAPDH as A ReferenceGene for Gene Expression Analysis ofEquine Adipose-Derived Mesenchymal Stem Cells

Background: Adipose tissue is a main source for isolation of equine mesenchymal stem cells (MSCs) at different ages. It seems that characteristics of adipose-derived MSCs especially gene expression profile are changing along with age increase. A proper reference gene is required for normalizing data in gene expression analysis by qRT-PCR. This study aimed to evaluate whether GAPDH has a stable ...

short Communication: Optimization of semi-quantitative RT PCR analysis for CPT I gene expression in Rainbow trout (Oncorhynchus mykiss)

Cloning, Codon Optimization, and Expression of Yersinia intermedia Phytase Gene in E. coli

Background: Phytate is an anti-nutritional factor in plants, which catches the most phosphorus contents and some vital minerals. Therefore, Phytase is added mainly as an additive to the monogastric animals’ foods to hydrolyze phytate and increase absorption of phosphorus. Objectives: Y. intermedia phytase is a new phytase with special characteristics such as high specific activity, pH stabilit...